Microscopic evidence for the uptake of orally given humic acids by the intestinal mucosa in piglets

Abstract

In veterinary medicine, humic acids are sometimes used as oral supplements to protect and treat young animals from diarrhoea. With regard to their mode of action, it was generally believed that humic acids are unable to penetrate the intestinal mucosal surface and rather act from the intestinal lumen. In the past, some reports indicated, however, that prophylactic and therapeutic effects of orally administered humic acids might not be confined to the lumen of the digestive tract. The present study used piglets to examine whether orally administered humic acids would be able to cross the intestinal barrier and if so, whether the humic acids would also be transported from the intestine to other regions of the body. The study was carried out with three 64-day-old piglets, two of which were bottle fed daily with 1 g humic acids/kg bodyweight and day for 2 weeks. The third piglet served as an unsupplemented control. At the end of the study, the piglets were slaughtered and 10 tissue specimens were collected from each piglet. Examination by light microscopy of unstained sections revealed the presence of humic acid particles in each tissue sample from both humic acid-treated piglets whereby no such deposits were found in any tissue of the control piglet. This demonstrated that the humic acids had indeed passed the epithelial barrier of the intestinal mucosa and had been transported to other body tissues. In most tissue samples the humic acid particles showed a distinct clustered distribution pattern.