Microscopic evaluation of the effect of low-level laser therapy on platelet-rich fibrin: A light microscopic histological study

Abstract

Background: This study evaluated and compared the concentration of platelets/leukocytes, fibrin matrix, and its distribution in nonlaser-treated platelet-rich fibrin (PRF) and laser-treated PRF. Recently, PRF has been used for regeneration of soft and hard tissue, in periodontics and oral surgery. Low-level laser therapy (LLLT) has been used for many therapeutic purpose of tissue healing. The use of PRF treated with LLLT has shown to enhance better healing potential. Materials and Methods: In this study, we have selected five healthy controls and collected 10 ml of blood from them. All blood samples were centrifuged, and from each blood sample, two PRF clots were formed. A total of ten samples obtained were divided into two groups. Each group had five samples. One group was without LLL treatment (Group I) and other was treated with LLL (Group II). All the ten samples were processed for light microscopic examination. Results: In both the groups, the PRF membrane showed a similar macroscopic structure and microscopic distribution. In the buffy coat region, the concentration of platelets–leukocyte aggregate was little higher in Group I. In Group II, the distribution pattern of fibrin clot was slightly different with that of Group I. Conclusion: The Choukroun's PRF concept leads to specific clot architecture with platelets and fibrin meshwork, especially in the buffy coat region. Changes in the PRF components with LLLT may have an affect the healing and regenerative of soft and hard tissues.