MICROSCOPIC CHANGES IN THE LIVER AND THE HEART OF QUAILS DURING THE EXPERIMENTAL TOXICOSIS OF IRON(IV) CLATROCHELATE

Abstract

Iron(IV) clatrochelate is a unique compound because it contains iron in a rare high valence ˗ IV. It is characterized by high stability at high temperatures, different pH values etc. The studies of acute and chronic toxicity of this compound, started for the first time in Ukraine, have the important theoretical and practical significance, as this complex can be recommended as an active substance in iron-containing drugs with antianemic action.
 We investigated the acute and chronic toxicity of Iron(IV) based on a macrocyclic ligand of the hexahydrazide type for white mice, white rats and quails. Iron(IV) clatrochelate corresponds to hazard class III according to the classification of chemicals by degree of danger (GOST 12.1.007-76), and class IV and degree of toxicity ˗ "low-toxic substances" according to the classification of substances by toxicity. Thus, the average lethal dose of Iron(IV) clatrochelate for quails when administered orally is 764.3 ± 32.71 mg / kg body weight.
 Preclinical studies of new drugs include microscopic studies. It was found that under conditions of experimental intoxication with Iron(IV) clathrochelate, quails develop pathological changes that correlate with the dose of the test compound. The higher the dose, the more severe the lesions. The available changes in the liver and heart on histological examination indicated a violation of metabolic processes in the body of quails of the experimental groups, which used Iron(IV) clatrochelate.
 This compound causes a significant disruption of protein metabolism, which during acute poisoning with this compound leads to the formation of areas of destruction and necrosis of the heart muscle, and during chronic ˗ to the granular dystrophy and partial destruction of muscle fibers sarcoplasm with disruption of their relationship. Microscopically noticeable changes do not occur in the endocardium and epicardium.