Abstract

Fifty three baladi rabbit embryos and fetuses were used in this study. The results revealed that the testicular differentiation occurred with the formation of the testicular cords with their constituents, gonocytes and primitive Sertoli cells at the 18<sup>th</sup> day postconception. The Sertoli cells were increased in number from 20<sup>th</sup> day postconception onwards and they appeared as small sized cells with oval darker nuclei. The Leydig cells were demonstrated at the 20<sup>th</sup> day of gestation life as clusters of polyhedral large cells with strongly acidophilic finely granular cytoplasm and large, vesicular, spherical and eccentric nuclei. At 28<sup>th</sup> day postconception up to the full term rabbit fetus, the Leydig cells of the testis showed a marked reduction in size and number.

Highlights

  • Testicles are the important organ for production of spermatozoa and testosterone

  • The testicular differentiation was evident by the formation of an incomplete tunica albuginea and primitive testicular cords which consisted of numerous primitive supportive cells together with gonocytes

  • In 25th day-old rabbit fetus up to the full term rabbit fetus, the supportive cells transformed into columnar-shaped cells with oval or elongated nuclei which most of them were perpendicular to the basement membrane of the testicular cord, while few of them were centrally situated. (Figures 3, 4)

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Summary

Introduction

Testicles are the important organ for production of spermatozoa and testosterone. The domestic rabbit is valued as a popular live laboratory animals used in laboratory researchers as it give offsprings monthly and copulation is necessary to initiate ovulation. It copulates readily and ovulates approximately 10 hours after copulation, so that allow accurate pregnancy timing [1]. Leydig cells are responsible for secretion of testosterone hormone which maintains spermatogenesis. The present study aimed to record normal structural changes in the prenatal developmental stages of Sertoli and Leydig cells in rabbit, so that it could be used as an aid to distinguish from altered cells on rabbits

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Results
Conclusion

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