Abstract
Assembly of a high numerical objective platform for surface plasmon resonance imaging (SPRI) was used to investigate a high resolution diffraction limited refractive index image of cell-substrate contacts. SPRI is a highly sensitive biochemical surface sensor measurement technique that has only recently been applied to the field of cell-biology. The nature of the refractive index measurement made at the cell-surface interface is investigated. We present SPRI measurements of several different cell types as well as multimode image comparisons with fluorescence and transmission mode microscopies to scrutinize the signal composition of the SPRI refractive index signal. While the primary correlation of the SPRI signal appears to be the cellular membrane distance to substrate, depending on the cell type, cell focal contacts, and cellular organelles can also contribute to subcellular refractive index changes at the cell-substrate interface.
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