Abstract
The germination of nylon net-trapped microsclerotia of Verticillium dahliae pathogenic to rape ( Brassica napus) was assessed in different systems by fluorescence microscopy using fluorescein diacetate. The influence of the culture's age and the size of the microsclerotia on germination percentages was assessed in water, mineral salts solution and mineral salts solution plus sucrose for 3 V. dahliae isolates. Large microsclerotia germinated better than smaller ones. The microsclerotia of 2 isolates showed decreased germination percentages with culture age over a 4–11-week period. Microsclerotial germination percentages were always higher in mineral salts solution plus sucrose than in mineral salts solution alone or water. In a sand culture system with the intact rape plant, microsclerotial germination percentages were high close to the root and decreased in a steep gradient to background levels within 5 mm from the root.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
