Microscale investigations of microbial communities in coastal surficial sediments

Abstract

AbstractThe upper millimeters of sediments are considered major locations of microbial colonization and activity. The goal of our study was to investigate variations of microbial communities in the uppermost sediment layers of the coastal Baltic Sea with emphasis on the complex interplay between microbiology and physico‐chemical sediment properties. We selected a high‐resolution methodological approach that combined the cryolanding sampling technique (spatial resolution of 250 μm) with microsensor‐, spectrophotometric and microscopic analyses. While the oxygen penetration depths in dark conditions ranged from 1.4 mm to 2.6 mm during the study period, this zone expanded by about 1 mm in light and could be divided into three micro‐horizons: (i) an upper zone with a high net O2 production, (ii) an intermediate zone with increased O2 consumption on account of light‐stimulated respiration and (iii) a lower zone with lower O2 consumption. Time‐series experiments revealed a rapid response of the benthic microbial community to altered light intensities. In May and July, the net O2 budget in the porewater of sediments was positive within 35 and 22 min after illumination, respectively, whereas in June O2 production exceeded O2 consumption after 112 min. The thickness of the O2 production and O2 consumption micro‐zones decreased from May to July coinciding with an increase in temperature. In May, sites of enhanced O2 consumption were closely associated with subsurface maxima of microbial numbers and enzymatic activities indicating a tight coupling between photoautotrophic and heterotrophic processes between 1‐ and 2‐mm depth. In June and July, the microbial abundance and enzymatic activity hardly varied with depth. Spatial and temporal microheterogeneity of microbial distribution and activity in O2 gradients was seen as a reflection of the complex interplay between microbiology and physico‐chemical sediment properties.