Microsatellites for coriander crop: A cross species amplification

Abstract

Development of microsatellites (Simple Sequence Repeats) is an expensive and time consuming process for new crop like coriander, but this can be easily achieved by transferring the microsatellite loci from the same family/genus/species which is a cost-effective approach for development of microsatellites in lesser studied crops like coriander. For cross-species investigation presently carrot and celery microsatellites are available on the public domain. We examined the transferability of hundred carrot (Daucuscarota) microsatellite loci to coriander (Coriandrum sativum L.). Thirty nine percent primer (39/100) amplified for coriander. Out of 39 microsatellites loci, 35 primers were monomorphic and specific to carrot primers, showing approximately the same size of microsatellites loci as reported for carrot. Four SSRs were polymorphic; seven primer pairs were non-specific which were deviating for band size from the source of microsatellites. These microsatellites markers should provide a powerful tool for coriander breeding and genetics.