Microsatellites and mitochondrial DNA reveal regional population structure in bobcats (Lynx rufus) of North America

Abstract

Genetic analyses can facilitate large-scale conservation planning for wide-ranging species capable of long-distance dispersal. Bobcats (Lynx rufus) are the most broadly distributed native felid in North America, and are managed on a “state-by-state” basis. Little is known about the distribution of genetic diversity across bobcats’ range. We examined genetic differentiation among bobcats from throughout their distributional range in North America using 10 microsatellite loci and mitochondrial control region sequence to elucidate patterns of genetic diversity. Both markers revealed significant regional genetic differentiation. Additionally, genetic diversity estimates, population expansion statistics, and the haplotype network elucidated from mitochondrial DNA analyses, suggest that populations in the West and East/Midwest experienced historical population expansions, with the East/Midwest likely undergoing periodic range contractions and extirpations during bobcat colonization. Microsatellite data revealed significant regional genetic differentiation between the Midwest and East, as well as the West, suggesting that recent barriers to gene flow may be affecting dispersal of bobcats. These analyses indicate that conservation of forested areas will be crucial for maintaining gene flow throughout bobcats’ range as human populations increase and that multi-state consortia may be a more appropriate way to manage bobcats as this scenario will conserve both historical and current levels of genetic diversity.