Abstract
Materials and methods The DNA was extracted from blood samples by using the standard phenol-chloroform extraction method and then amplified by PCR using specific primers for TPOX locus. The PCR products were separated by electrophoresis on denaturing 6% polyacrylamide gel and silver staining was done to resolve and observe different alleles. The observed and expected heterozygosity (Hobs and Hexp) as well as forensic and paternity indices including matching probability (MP), power of discrimination (PD), power of exclusion (PE) and polymorphism information content (PIC) were calculated using the Power Stats v.1.2 software for TPOX locus of the studied population. The POPGENE (v.32) statistical package was used for analyzing allele frequency.
Highlights
Autosomal short tandem repeats (STRs) have become the most informative molecular markers because they are highly polymorphic and multiallelic
Materials and methods The DNA was extracted from blood samples by using the standard phenol-chloroform extraction method and amplified by PCR using specific primers for TPOX locus
The PCR products were separated by electrophoresis on denaturing 6% polyacrylamide gel and silver staining was done to resolve and observe different alleles
Summary
Microsatellite variation and allele frequency distribution for (TPOX) STRS locus in North Indian Muslim populations. From International Conference on Human Genetics and 39th Annual Meeting of the Indian Society of Human Genetics (ISHG) Ahmadabad, India. 23-25 January 2013
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