Microsatellite markers for population-genetic studies of shiitake (Lentinula edodes) strains

Abstract

In order to analyze the genotyping of shiitake (Lentinula edodes, Berk.), one of commercially and widely grown edible mushrooms, we examined group of total 89 strains that are registered in Korea, Japan, and China respectively, using five microsatellite markers (Led A2, Led A8, Led B2, Led B6, and Led D6) registered in NCBI (National Center for Biotechnology Information). After we prepared synthesis of primers modified with 5′-FAM fluorescent dye and conducted PCR program, we obtained reasonable products. And then we performed microsatellite genotyping analysis using an ABI 3730xl Genetic Analyzer. According to genotyping analysis, the number of alleles of each microsatellite marker ranged from 5 to 14 with the average value of 8.2. The expected and the observed heterozygosity over all microsatellite ranged from 0.27–0.83 and 0.14–0.61. Among 5 microsatellite markers PIC (Polymorphism Information Content) values of Led A8, Led D6, and Led B6 resulted 0.82, 0.60, and 0.57 respectively. We observed that these values showed relatively high values discriminating from other values of microsatellite marker. The average of total values of Led A8, Led D6, and Led B6 came out 0.53 as result, which is higher than 0.5. As a result, this average can be subject to significant value to be used as marker. By using microsatellite marker we can analogically establish population relationships among shiitake (L. edodes) strains grown in East Asian region, develop new varieties, and propose discriminative criteria for different breeding and variety classification. Moreover, microsatellite markers enable us to obtain the genetic inheritance and information that can be used for protection of intellectual property rights.