Abstract
Population genetics of the coral genus Pocillopora have been more intensively studied than those of any other reef-building taxon. However, recent investigations have revealed that the current morphological classification is inadequate to represent genetic lineages. In this study, we isolated and characterized novel microsatellite loci from morphological Pocillopora meandrina (Type 1) and Pocillopora acuta (Type 5). Furthermore, we characterized previously reported microsatellite loci. A total of 27 loci (13 novel loci) proved useful for population genetic analyses at two sites in the Ryukyu Archipelago, in the northwestern Pacific. Clonal diversity differed in each genetic lineage. Genetic structure suggested by microsatellites corresponded to clusters in a phylogenetic tree constructed from a mitochondrial open reading frame (mtORF). In addition, we found an unknown mitochondrial haplotype of this mtORF. These microsatellite loci will be useful for studies of connectivity and genetic diversity of Pocillopora populations, and will also support coral reef conservation.
Highlights
The genus Pocillopora, especially Pocillopora damicornis, is the most studied reef-building coral in terms of population genetics[1,2,3,4,5,6,7,8,9]
16 or more genetic lineages of Pocillopora have been considered as putative species based on haplotypes of a mitochondrial DNA region[10, 12, 15] and genetic structure analyzed using microsatellite markers[10, 15]
In this study, using next-generation sequencing, we developed cross-lineage microsatellite loci in order to facilitate population genetic analyses for multiple lineages of Pocillopora in the Ryukyu Archipelago
Summary
Received: 29 March 2017 Accepted: 16 June 2017 Published online: 27 July 2017 populations. We validated and characterized microsatellites previously reported from Pocillopora for populations in the Ryukyu Archipelago Those were developed by Magalon et al.[20], Magalon et al unpublished, and Starger et al.[21] and recently, more loci have been isolated by Pinzón & LaJeunesse[13] and Torda et al.[8]. Gorospe & Karl[22] re-designed primers for two loci, PV7 from Magalon et al.[20] and Pd3-010 from Starger et al.[21] and designated them as Pd2-AB79 and Pd3-EF65, respectively Using all of these validated microsatellite markers, we genetically characterized populations from multiple lineages of Pocillopora from Ueno and Yoshino, Miyako Island, in the Ryukyu Archipelago
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