Microsatellite markers for identification of a group of italian olive accessions

Innocenzo Muzzalupo,Francesca Stefanizzi,Rosanna Falabella,Amelia Salimonti,Enzo Perri

doi:10.1590/s0103-90162009000500014

Innocenzo Muzzalupo, Francesca Stefanizzi + Show 3 more

Open Access

https://doi.org/10.1590/s0103-90162009000500014

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Abstract

Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.

Highlights

The species Olea europaea includes several subspecies with different morphological traits and geographical origins (Green, 2002)
The Italian olive germplasm is estimated to include over 680 accessions and at least 1300 synonyms (Bartolini et al, 2005; Fiorino et al, 2005), most of which are landraces vegetatively propagated at the farm level since ancient times
Except for UDO9901, which was monomorphic in all the olive accessions analyzed

Summary

Introduction

The species Olea europaea includes several subspecies with different morphological traits and geographical origins (Green, 2002). The systematic collection and subsequent description and characterization of Italian olive cultivars in specific catalogue fields began in Italy in the 1980s while a nationwide collection was begun in 1997 by CRA-OLI of Rende. The goal of such collections is to safeguard all cultivars, and the minor ones, to avoid a loss in genetic diversity and to offer an interesting genetic basis for breeding programs. Large efforts have been made in characterizing olive germplasms using morphological and agronomic traits (Lombardo et al, 2004; Khadari et al, 2007) and/or different types of biochemical and molecular markers (Muzzalupo & Perri, 2008).

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