Abstract
The python of Indonesian Sundaland has been traded for its distinct skin colour and patterns. The need for rapid method in cross-species amplification for Indonesian Sundaland python is useful to contribute in management of sustainability harvesting system. In this research, we screened 10 microsatellite primers which are previously used for Australian, New Guinean, Chinese and Burmese pythons and 7 potentially amplifiable primers for African and Asian reptiles. Python breitensteini showed a greater number of alleles (2-8 alleles) than Python bivittatus (1-3 alleles) and Python brongersmai (1-2 alleles). The observed and expected heterozygosity for all species were ranged from 0 to 1.00 and 0 to 0.79, respectively. According to the high cross-species amplification rates, 15 out of 17 primers were useful in assessing the genetic diversity and conservation genetic of Indonesian Sundaland python. Among the 15 primers, MS3 generated the highest number of allele for P. breitensteini (8 alleles), P. bivittatus (3 alleles), and P. brongersmai (2 alleles). We proposed MS3 locus as a suitable marker for Indonesian Sundaland python. Keywords: microsatellite, Python, Sundaland.
Highlights
INTRODUCTIONSubspecies, P. reticulatus reticulatus which was widely distributed in Sumatra, Kalimantan, Java, and Sulawesi
Python is a nonvenomous snake and famous for its large body size
We proposed MS3 locus as a suitable marker for Indonesian Sundaland python
Summary
Subspecies, P. reticulatus reticulatus which was widely distributed in Sumatra, Kalimantan, Java, and Sulawesi. The trade of two other subspecies, P. reticulatus saputrai (Selayar Island and Southwest Sulawesi) and P. reticulatus jampeanus (Tanahjampe Island), were banned according to Indonesian annual quota system. The use of microsatellite markers has been widely developed to detect illegal trade in many species such as broad-headed snakes, Hoplocephalus bungaroides [5], Cape parrot, Poicephalus robustus [6], and the identification of ivory species origin traded in African black market [7]. The development of microsatellite primers may cost considerable price and time consuming, in order that the use of cross-species amplification becomes an alternative way to find a suitable marker for closely related species determination
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