Microsatellite marker based characterization of mango cultivars

Abstract

Simple sequence repeats (SSRs) or microsatellites are highly efficient in the classification of genotypes, genetic resources utilization and breeding programmes. In the present investigation, seventeen (17) SSR primers were used for the molecular characterisation of twenty mango cultivars of Bihar. Out of 58 scorable bands, 45 were found to be polymorphic. The number of alleles detected ranged from 2 (MIAC 5, MiIIHR 12, MiIIHR 20a, MiSHRS 37) to 6 (MIAC 11, MiIIHR06, mMiCIR 027). The polymorphic information content (PIC) ranged from 0.05 (MiIIHR 20a) to 0.38 (MIAC 2). The genetic relationship among twenty mango cultivars based on Jaccard's similarity coefficient was found to be ranging from 0.46 (between Fazli and Langra) to 0.90 (between Krishnabhog and SB Chausa and Krishnabhog and Bangalora).The dendrogram based on UPGMA cluster analysis grouped twenty mango cultivars into two major clusters while the cultivars Neelum and Fazli were found to be distinct from other cultivars. SSR markers are reliable and reproducible and have been proved to be useful for varietal identification and mango breeding programmes to maximize genetic variability among the mango cultivars. The results of this study have showed the potential of SSR markers in deciphering the existing genetic diversity among the Indian mango cultivars.