Microsatellite marker assisted molecular diversity and population structure analysis of Kaempferia galanga Linn. germplasm collected from different parts of India

Abstract

Kaempferia galanga Linn. is an endangered medicinal and aromatic crop with a wide range of importance in the field of pharmaceutical industries. The current study is sought to examine the molecular variance and population diversity for the first time within the core collection of K. galanga germplasm. For this study, a total of 132 accessions of K. galanga were collected from various states of India and all the germplasm has been maintained ex-situ at the experimental farm of CSIR-NEIST, Jorhat. Initially, 58 simple sequence repeats (SSR) markers were tested among them 48 markers that were the most efficient, sensitive, with positive predictive and polymorphism were used in the study. The population structure analysis of K. galanga showed nine clusters based on the Bayesian model. The estimated population heterozygosity ranged from 0.117 to 0.250. According to the Analysis of molecular variance study (AMOVA), the variation within the population (68%) was more than among the population (32%). The genetic diversity among the population (Fst) was in the range of 0.28–0.60. The accessions under study demonstrated a high amount of polymorphism with a 99.4% polymorphism observed in molecular investigation. The outcome of the study would help in the identification of the genetic variance for unique germplasm which would be of great aid in the breeding program of K. galanga.