Abstract
This study was performed for parentage verification of the Korean native horse (KNH). 103 random KNH samples (including 19 foals for parentage testing) were genotyped by using 16 microsatellite markers. The number of alleles per locus varied from 5 to 13 with an average value of 8.56 in the KNH. The observed heterozygosity and the expected heterozygosity ranged 0.398-0.893 (the average value was 0.683) and 0.368-0.871 (the average value was 0.727) in the KNH, respectively. The PIC value and the exclusion probability ranged 0.347-0.853 (the average value was 0.692) and 0.208-0.736, respectively, and the total exclusion probability of 16 microsatellite loci was 0.9999. Of the 16 markers, AHT4, AHT5, ASB2, ASB17, HMS2, HMS3, HTG10, LEX33, TKY321 and VHL20 loci have a relatively high PIC value (>0.7) in the KNH. Of the 19 foals, 5 foals were disqualified by an incompatibility of 4-7 markers according to a Mendelian fashion in the present DNA typing for parentage testing. These results present basic information for developing a system for parentage verification and individual identification in the KNH.
Highlights
The term microsatellites, which are known as short tandem repeats, refers to a class of codominant DNA markers which are inherited in a Mendelian fashion.Microsatellite loci are widely dispersed along and among choromosomes and each locus is characterized by a knownDNA sequence
Genomic DNAs were prepared from whole blood samples, which were collected from 103 Korean native horse (KNH) including 19 samples used for parentage testing
All of the primers amplified very well, with the exception of HTG10 that sometimes exhibited low peak heights, especially the L allele, this allele being unsuitable for automated scoring
Summary
They are typically composed of between two to four nucleotides such as (CA)n or (GATA)n where n lies between 5 and 50 (Dewoody and Avise, 2000). The Korean native horse (KNH) is one of the Korean native animals which was designated by the government as a natural monument No 347 on February 1985 (Cho et al., 2001). They have been separated into distinct areas and partly, they have been used as a racehorse in the Jeju race course of Korea Racing Association. Jeju Island is the major province for producing the Thoroughbred (T.B) in
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