Abstract
The genetic variability of three poultry breeds namely Aseel, Miri and Nicobari taken from different geographical locations of India were evaluated using 15 microsatellite loci. No. of alleles varied from 3 to 9 in Aseel,3 to 8 in Miri and 2 to 7 in Nicobari. Mean PIC values in Aseel, Miri and Nicobari breeds were 0.64, 0.66 and 0.63, respectively. Average unbiased heterozygosity and direct count heterozygosity were 0.65 and 0.59, 0.68 and 0.61, and 0.64 and 0.57 in Aseel, Miri and Nicobari breeds, respectively. High heterozygosity values revealed in this study are indicative of low level of inbreeding, large population size and no or low selection pressure for commercial trait in all three populations. The estimate of genetic distances using Nei's standard, Nei's minimum and Reynold's distance revealed Aseel and Nicobari to be more closely related than Miri breed of poultry.
Highlights
There are 17 defined breeds of domestic fowl (Gallus domesticus) in India (Acharya and Bhat, 1984)
The three diverse breeds from different geographical location Aseel, Miri and Nicobari were identified for the study
These breeds are of local importance in their regions and are reared as backyard poultry
Summary
There are 17 defined breeds of domestic fowl (Gallus domesticus) in India (Acharya and Bhat, 1984). The three diverse breeds from different geographical location Aseel, Miri and Nicobari were identified for the study. These breeds are of local importance in their regions and are reared as backyard poultry. Several techniques like RAPD, AFLP and Microsatellite analysis are utilized to assess the population relationships. Among these the microsatellite analysis provide even distribution in the genome, and are highly polymorphic and are markers of choice for biodiversity analysis. In this study 15 highly polymorphic unlinked microsatellite markers have been utilized for genetic relationship among three native breeds of India
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