Abstract
Assessment of genetic variability in Kangayam breed of cattle in Tamilnadu, South India was carried out using 25 bovine microsatellite markers. The mean number of alleles was 4.04 ± 0.09 with a range of 2 to 6 and the allele size ranged from 94 to 300 bp. The frequency distribution of alleles in the breed was from 0.0104 to 0.9167. The estimated heterozygosity was 0.6183 ± 0.01 and the PIC was 0.5628 ± 0.03. The overall mean within-population inbreeding estimate (FIS) value (- 0.084) suggested excess of heterozygotes in the population. In addition, higher PIC value indicated the scope for maintaining variation in the population and strategies to take meaningful conservation.
Highlights
The allele size ranged from 94 to 300 bp
The Kangayam breed of cattle of Tamilnadu is best polymerase (Invitrogen, USA) and 100mM dNTPs was known for its superior draught qualities, adaptation to prepared
The Kangayam cattle is distributed in Erode, Dindigul, Karur, Coimbatore, Salem and Namakkal districts of Tamilnadu state in South India
Summary
The allele size ranged from 94 to 300 bp. The PCR amplification and microsatellite analysis frequency distribution of alleles in the breed was from. A total number of 101 alleles were observed in these polymorphic loci in the breed. Umblachery cattle, the other draught breed of cattle in Tamilnadu (karthickeyan et al, Table 1 Allele frequency, polymorphism information content (PIC) and heterozygosity of microsatellite loci in Kangayam breed of cattle
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