Abstract
Background. Systemic glucocorticoids are prohibited in-competition by the World Anti-Doping Agency. Here, we describe an original microsampling workflow for the quantitation of three endogenous (cortisol, corticosterone andcortisone) and three exogenous (dexamethasone, methylprednisolone andfludrocortisone) corticosteroids in 30μl of human urine. Materials & methods. Microsampling was carried out by dried urine spot(DUS) samplingand volumetric absorptive microsampling (VAMS), followed by solvent extraction and LC-MS/MS analysis. Results & conclusion: Good linearity (r2> 0.9989) was obtained for all analytes; extraction yields (>81%), precision (RSD<8.6%) and matrix effect (<12%) were satisfactory. Microsample stability at room temperature was good (analyte loss<15% after 3 months). Data obtained from real urine microsample analysis were compared with those of fluid urine, providing very good agreement (r2> 0.9991).
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