Abstract
A comprehensive analysis of the neuroanatomical expression profiles of 38 abundant conserved miRNAs in developing and adult zebrafish brain was performed.
Highlights
MicroRNA encoding genes are abundant in vertebrate genomes but very few have been studied in any detail
In order to survey the expression patterns of miRNAs in the brain, we performed in situ hybridizations with locked nucleic acid (LNA) probes to 38 different miRNAs (Table J in Additional data file 28,) at 3 and/or 5 days and/or 6 weeks (young adult ('Y-Ad' in Additional data files 1-29)) and/or adult zebrafish (adult ('A' in Additional data files 1-29))
To examine if the LNA probes can discriminate between miRNAs having only one or more different nucleotides, we performed in situ hybridization for four miRNAs using one or two internal mismatches (Table L in Additional data file 28, and Additional data file 29)
Summary
MicroRNA (miRNA) encoding genes are abundant in vertebrate genomes but very few have been studied in any detail. The central nervous system (CNS) is a prominent site of miRNA expression, virtually nothing is known about the spatial and temporal expression profiles of miRNAs in the brain. The precise mechanism of miRNA-mediated gene silencing remains uncertain, miRNAs can promote de-adenylation that likely destabilizes mRNAs, leads to their clearance [6,7,8] and/or induces translational repression of target mRNAs [9,10]. Target mRNAs are usually transcribed at low levels whenever their targeting miRNAs are expressed [11,12]. This complementarity can occur through spatial or temporal reciprocity of miRNA and target mRNA gene expression. Other roles for miRNAs are likely, and may involve contemporaneous expression and function of miRNAs and their targets [13,14]
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