MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis.

Chihiro Kiyoshima,Kenichi Yoshikawa,Daisuke Izuchi,Makoto Nomiyama,Tetsuya Hayashi,Yoshitoshi Ogura,Satoshi Fukagawa,Kenichiro Hata,Kazuki Nabeshima,Fusanori Yotsumoto,Ohta Eiji,Shingo Miyamoto,Daichi Urushiyama,Naoto Shirasu,Kenji Hiromatsu,Masamitsu Kurakazu,Shin’ichiro Yasunaga ,Sachiko Hirose ,Tsubasa Hirakawa ,Koichiro Miyata

doi:10.2144/fsoa-2021-0006

Abstract

Background:This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis.Materials & methods:Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc’s criteria.Results:The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation.Conclusion:miR-4535 and miR-1915-5p serve as promising biomarkers for the diagnosis of severe chorioamnionitis.

Highlights

This study was performed to investigate the clinical significance of miR-4535 and miR-19155p in severe chorioamnionitis
• Increasing average age of women at childbirth is associated with an increased incidence of premature birth
• Chorioamnionitis is associated with a range of morbidities including premature birth

Summary

Introduction

This study was performed to investigate the clinical significance of miR-4535 and miR-19155p in severe chorioamnionitis. Materials & methods: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCRTM. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc’s criteria. Results: The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation. Conclusion: miR-4535 and miR-1915-5p serve as promising biomarkers for the diagnosis of severe chorioamnionitis

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Discussion

Conclusion