Abstract
MicroRNAs (miRNAs) are associated with the initiation and progression of oral squamous cell carcinoma (OSCC) by regulating a variety of cancer‑associated behaviors. Fully understanding the regulatory mechanism of miRNAs in the pathogenesis of OSCC may provide novel promising approaches for the identification of prognostic biomarkers and therapeutic targets for this particular malignancy. In the present study, reverse transcription‑quantitative polymerase chain reaction analysis was performed to detect miRNA (miR)‑495 expression in OSCC tissues and cell lines. The effects of miR‑495 on the proliferation and invasion of OSCC cells were determined using Cell Counting Kit‑8 and Matrigel invasion assays, respectively. The mechanisms underlying the action of miR‑495 in OSCC cells were also investigated. Results from the present study revealed that miR‑495 expression was downregulated in OSCC tissues and cell lines compare with in adjacent normal tissues and human oral keratinocytes, respectively. Exogenous expression of miR‑495 restricted cell proliferation and invasion of OSCC cells invitro. Notch1 was identified as a direct functional target of miR‑495 in OSCC. Furthermore, Notch1 knockdown exhibited inhibitory effects, similar to those induced by miR‑495 overexpression in OSCC cells. Restoration of Notch1 expression rescued the suppressive effects of miR‑495 on OSCC cell proliferation and invasion. These findings suggested an important role for miR‑495 in the regulation of OSCC cell growth and metastasis, at least partly by directly targeting Notch1. In addition, the findings of the present study revealed the potential of miR‑495 as a novel therapeutic target for the treatment of patients with OSCC.
Highlights
Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck neoplasms, and accounts for ~3% of all recently diagnosed tumor patients [1]
Results from Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) revealed that miR‐495 expression levels were significantly lower in OSCC tissues compared with the expression levels in the adjacent normal tissues (P
Discussion miRNAs have been associated with the initiation and progression of OSCC by regulating a variety of cancer‐associated behaviors, including cell proliferation, apoptosis, cell cycle, invasion, metastasis and angiogenesis [32,33,34]
Summary
Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck neoplasms, and accounts for ~3% of all recently diagnosed tumor patients [1]. Despite considerable advances in OSCC treatment, the prognosis of patients with OSCC has demonstrated no notable improvements in recent decades [2]. The poor prognosis of this disease is mainly due to late stage diagnosis, radiation resistance and recurrence; distant metastases have been reported following combined treatment regimens [4,5]. The occurrence and development of OSCC is a complex process involving numerous genetic and epigenetic alterations and dynamic alterations in the expression of coding and non‐coding RNAs [6,7,8]; the specific mechanism underlying the pathogenesis of OSCC remains unknown. An enhanced understanding of the mechanisms underlying the carcinogenesis and progression of OSCC is critical to the development of novel and effective therapeutic methods to improve the treatment outcomes of this disease
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