MicroRNA-492 overexpression exerts suppressive effects on the progression of osteosarcoma by targeting PAK7.

Xuanhe Song,Yaoping Xie,Ming Shao,Yang Liu,Weiliang Yang

doi:10.3892/ijmm.2017.3046

Abstract

MicroRNAs(miRNAs or miRs), which are a class of non-coding RNAs, have emerged as effective modulators of various aspects of biological processes. Accumulating evidence has established significant associations between the dysregulation of miRs and tumorigenesis in various types of cancer. However, the role of miR‑492, particularly in osteosarcoma(OS) remains elusive. In present study, we demonstrated that miR‑492 functions as putative tumor suppressor miR in OS. The level of miR‑492 was frequently downregulated in both OS tissues and cell lines. Moreover, the ectopic overexpression of miR‑492 effectively inhibited the proliferation, migration and invasion of OS cell lines. Furthermore, transfection with a miR‑492 overexpression vector also strongly attenuated the growth of xenograft tumors invivo. p21-activated kinase(PAK7) was identified as the putative target of miR‑492 in OS, and we further found a significantly inverse correlation between PAK7 and miR‑492 in OS specimens. Taken together, our study has unraveled a novel role for miR‑492 in OS and may help in establishing the rationale for more effective treatment strategies for OS via miR regulation.

Highlights

Tumorigenesis has been a serious threat to human health and survival for decades and among the malignant cancers, osteosarcoma (OS) is one of the most common tumors, in adolescents [1]
Expression of miR‐492 is reduced in OS samples and cell lines
The results revealed that the level of miR‐492 was significantly downregulated in the OS samples compared with the corresponding paired normal adjacent tissues (n=77, P

Summary

Introduction

Tumorigenesis has been a serious threat to human health and survival for decades and among the malignant cancers, osteosarcoma (OS) is one of the most common tumors, in adolescents [1]. The occurrence of OS denotes a complex mechanism where multiple factors from environmental and genetic origins may largely contribute to the complexity [2]. During the development of OS, immature bones are formed and normal bones are jeopardized [3]. Long bones are at a greater risk of the onset of OS, those in The in-depth understanding of the occurrence and development of OS poses a new challenge to biological researchers

Methods

Results

Conclusion