Abstract
The increasing evidences have revealed that microRNAs (miRNAs) play significant role in nutrient stress response. Previously, miR399 was documented to be induced by phosphorus (P) starvation and involved in regulating P starvation responses. To further investigate the function of miR399 in rice (Oryza sativa L.), we performed GeneChip analysis with OsmiR399 over-expressing plants. Interestingly, our results showed that, besides P starvation responsive genes, the expression of a number of genes involved in iron (Fe), potassium (K), sodium (Na), and calcium (Ca) absorption was dramatically up-regulated in OsmiR399 over-expressing plants. Consistently, the concentrations of Fe, K, Na, and Ca were also increased in OsmiR399 over-expressing plants. The expression of OsmiR399 was also up-regulated by these nutrient starvations, respectively. Moreover, the loss-of-function of LTN1, the down-stream target of OsmiR399, also resulted in the increase of multiple metal elements and the up-regulation of the absorption related genes. These results indicated that OsmiR399 participates in the regulation of multiple nutrient starvation responses, which also gives new view on understanding the interaction among different nutrients mediated by miR399.
Highlights
MicroRNA is a class of 21–24 nucleotides no-coding small RNA, which usually downregulates the expression of target genes at post-transcription level through mRNA degradation or translational repression
The expression of Pi absorption-related genes was significantly increased in the roots of OsmiR399-ox plants (Table 1)
Several genes probably involved in metal element absorption were largely up-regulated in OsmiR399-ox plants (Table 1), including the genes encoding nicotianamine synthase (OsNAS1 and OsNAS2), potassium transporter (KT), sodium transporter (NaT), and calcium channel (CaC), which gives the hint for the connection between miR399 and multiple nutrient absorption in rice
Summary
MicroRNA (miRNA) is a class of 21–24 nucleotides no-coding small RNA, which usually downregulates the expression of target genes at post-transcription level through mRNA degradation or translational repression. The stress-regulated miRNAs have been identified under different stresses, such as drought, cold, salinity, and nutrient deficiency. A great number of miRNAs, for example, miR396, miR168, miR167, miR165, miR319, miR159, miR394, miR156, miR393, miR171, miR158, and miR169 have been identified to be regulated by drought in Arabidopsis (Liu et al, 2008). The expression of miRNAs is differentially regulated by salt. In Arabidopsis, miR156, miR158, miR159, miR165, miR167, miR168, miR169, miR171, miR319, miR393, miR394, miR396, and miR397 are up-regulated by salt stress while the expression of miR398 is down-regulated (Liu et al, 2008). The expression of miRNAs under cold or heat stress was studied in
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