Abstract
Osteosarcoma (OS) is an aggressive malignant mesenchymal neoplasm amongst adolescents. The aim of the present study was to explore the various modes of action that miR-379 has on the proliferation, migration, and invasion of human OS cells. miR-379 achieves this by targetting eukaryotic initiation factor 4GII (EIF4G2). Human OS cell lines U2OS and MG-63 were selected and assigned into blank, miR-379 mimics, miR-379 mimic negative control (NC), miR-379 inhibitors, miR-379 inhibitor NC, EIF4G2 shRNA, control shRNA, and miR-379 inhibitor + EIF4G2 shRNA group. The miR-379 expression and EIF4G2 mRNA expression were detected utilising quantitative real-time PCR (qRT-PCR) and the EIF4G2 protein expression using Western blotting. MTT assay, scratch test, Transwell assay, and flow cytometry were performed to determine the proliferation, migration, invasion, and cell cycle, respectively. In comparison with the miR-379 mimic NC group, the miR-379 mimics group had decreased EIF4G2 expression; the miR-379 inhibitors group indicated an increased EIF4G2 expression. Compared with the control shRNA group, the EIF4G2 expression was lower in the EIF4G2 shRNA group and the miR-379 expression was dropped in the miR-379 inhibitor + EIF4G2 shRNA group. The proliferation, migration, and invasion abilities of OS cells were reduced in the miR-379 mimics and EIF4G2 shRNA groups. The percentage of OS cells at the G0/G1 stage was increased, and the percentage at the S-stage was decreased in the miR-379 mimics and EIF4G2 shRNA groups. miR-379 may inhibit the proliferation, migration and invasion of OS cells through the down-regulation of EIF4G2.
Highlights
Osteosarcoma (OS) is the most common primary malignancy and is derived from the primitive bone-forming mesenchymal cells in the long bones [1]
The results collected from quantitative real-time PCR (qRT-PCR) as well as Western Blotting (Figure 1) indicated that in the U2OS and MG-63 cell lines, no significant differences were found in the expressions of miR-379 and eukaryotic initiation factor 4GII (EIF4G2) amongst the miR-379 mimic negative control (NC), miR-379 inhibitor NC, control shRNA and blank groups
In comparison with the control shRNA group, the EIF4G2 expression was lower in the EIF4G2 shRNA group (P0.05) The miR-379 expression was dropped in the miR-379 inhibitor + EIF4G2 shRNA group (P0.05)
Summary
Osteosarcoma (OS) is the most common primary malignancy and is derived from the primitive bone-forming mesenchymal cells in the long bones [1]. OS commonly occurs in adolescents between the ages of 10 and 20, while accounting for 8.9% of cancer deaths amongst children and adolescents in the United States alone. The most common treatment available for OS is preand post-operational chemotherapy in association with surgical treatment [3]. Universal common risk factors linked to OS development include ionising radiation, alkylating agents, Paget’s disease, hereditary retinoblastoma, the Li–Fraumeni familial cancer syndrome and other chromosomal abnormalities [2]. Genetic aberrations have received increasing recognition as a key factor in the etiology of OS [2]
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