Abstract
Host innate immunity is the major player against continuous microbial infection. Various pathogenic bacteria adopt the strategies to evade the immunity and show resistance toward the various established therapies. Despite the advent of many antibiotics for bacterial infections, there is a substantial need for the host-directed therapies (HDTs) to combat the infection. HDTs are recently being adopted to be useful in eradicating intracellular bacterial infection. Changing the innate immune responses of the host cells alters pathogen’s ability to reside inside the cell. MicroRNAs are the small non-coding endogenous molecules and post-transcriptional regulators to target the 3’UTR of the messenger RNA. They are reported to modulate the host’s immune responses during bacterial infections. Exploiting microRNAs as a therapeutic candidate in HDTs upon bacterial infection is still in its infancy. Here, initially, we re-analyzed the publicly available transcriptomic dataset of macrophages, infected with different pathogenic bacteria and identified significant genes and microRNAs common to the differential infections. We thus identified and miR-30e-5p, to be upregulated in different bacterial infections which enhances innate immunity to combat bacterial replication by targeting key negative regulators such as SOCS1 and SOCS3 of innate immune signaling pathways. Therefore, we propose miR-30e-5p as one of the potential candidates to be considered for additional clinical validation toward HDTs.
Highlights
Variety of commensal bacteria were considered beneficial to human host and their role is crucial for the host survival
This analysis holds valid presumptions to be taken into consideration as the target cells used here were macrophages from healthy volunteers infected with bacteria (Listeria and Salmonella), are the crucial innate immune cells to play an important role in defense during bacterial infections
We further focused on miRNA-30e characterization as the host regulatory microRNA and concluded that miR-30e-5p induced upon different bacterial infections and stimulation with bacterial PAMPs in hPBMCs, HeLa and Raw264.7 cells respectively (Figures 1E–H)
Summary
Variety of commensal bacteria were considered beneficial to human host and their role is crucial for the host survival. Several bacteria qualify the category of potential pathogens to cause serious health ailments in humans ranging from the food-borne illnesses caused by species such as Listeria monocytogenes and Salmonella typhi, as well as tuberculosis caused by Mycobacterium tuberculosis and associated with oncogenesis. The alarming elevation of the antibacterial resistance against any bacterial disease possess biggest global threat and is a critical cause for the millions of human deaths annually around the world (Laxminarayan et al, 2013). Bacteria can cause outbreak, leading to sever miR-30e Regulates Bacterial Infection health damage and lives. A food-borne- bacteria Listeria monocytogenes caused an outbreak in South Africa leading to severe illness and deaths among the population (de Noordhout et al, 2014; Allam et al, 2018; Desai et al, 2019; Thomas et al, 2020). Re-exploring the host factors against bacterial infections might is needed
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
