MicroRNA‑28 promotes the proliferation of non‑small‑cell lung cancer cells by targeting PTEN.

Abstract

Non-small-cell lung cancer (NSCLC) is the fundamental form of lung cancer and the leading cause of cancer‑related mortality in humans. Numerous studies have identified a role for microRNAs (miRs) in cell proliferation, invasion and metastasis in numerous types of cancer, including lung cancer. In the present study, the functional roles and molecular mechanisms of miR‑28 in NSCLC tumorigenesis were investigated. Reverse transcription‑quantitative PCR (RT‑qPCR) was used to measure miR‑28 expression levels in NSCLC tumor tissues and cell lines. A dual‑luciferase assay was performed to observe the direct interaction between miR‑28 and PTEN in A549cells. Furthermore, the effect of miR‑28 on the mRNA and protein expression levels of PTEN was examined by RT‑qPCR and western blotting, respectively. A Cell Counting kit‑8 assay was performed to identify the relationship between the miR‑28/PTEN axis and tumor cell proliferation using cells infected with lentivirus (LV)‑anti‑miR‑28 or LV‑anti‑miR‑28+short hairpin RNA‑PTEN. miR‑28 expression was upregulated in NSCLC tumor tissues and cell lines compared with the control groups. PTEN was identified as the downstream gene of miR‑28 in NSCLC and was negatively regulated by miR‑28. In addition, miR‑28 knockdown suppressed the proliferation of A549 and H292cells. Cells infected with LV‑anti‑miR‑28+short hairpin RNA‑PTEN promoted tumor cell proliferation in A549 and H292cells compared with cells infected with LV‑anti‑miR‑28. Taken together, the present study suggested that miR‑28 might serve as the promoter in the development of NSCLC by targeting PTEN. Therefore, the miR‑28/PTEN axis may serve as a potential diagnostic and therapeutic target for NSCLC.