Abstract
MicroRNA (miR)-27b has been reported to participate in glioma. However, a detailed role of miR-27b and the underlying mechanism remain largely unknown. The present study found that the expression of miR-27b was significantly increased in glioma tissues compared with normal adjacent tissues. In addition, miR-27b was also upregulated in the U87, U251 and SHG44 glioma cell lines compared with normal human astrocytes. Sprouty homolog 2 (Spry2), which has been reported to be associated with invasive glioma, was identified as a novel target of miR-27b in U251 glioma cells, and the protein expression of Spry2 was negatively regulated by miR-27b in U251 cells. Additionally, inhibition of miR-27b and upregulation of Spry2 suppressed glioma cell invasion, while downregulation of Spry2 reversed the suppressive effect of miR-27b inhibition on glioma cell invasion. These data suggest that miR-27b may promote glioma cell invasion through direct inhibition of Spry2 expression. The data also suggest that miR-27b may become a promising molecular target for inhibiting the invasion and metastasis of glioma.
Highlights
Glioma is among the most common human malignancies in the brain
To the best of our knowledge, the roles of and association between miR‐27b and Sprouty homolog 2 (Spry2) have never been studied in glioma
It was revealed that the expression level of miR‐27b was markedly increased in glioma tissues and the U87, U251 and SHG44 glioma cell lines compared with normal brain tissue and astrocytes
Summary
Glioma is among the most common human malignancies in the brain. The five‐year survival rate of patients with glioma has been improved in recent years due to the combination of surgery, radiotherapy and chemotherapy, the prognosis of patients with invasive glioma remains poor [1,2]. It has been demonstrated that dysfunction of oncogenes or tumor suppressors is closely associated with the development and progression of glioma [2]. Developing novel molecular targets may be promising for the development of therapeutic strategies for invasive glioma. MicroRNAs (miRNAs), a class of non‐coding RNAs 18‐25 nucleotides in length, can induce mRNA degradation or The detailed role of miR‐27b in the regulation of invasive glioma remains largely unknown
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
