Abstract
Ovarian cancer is prone to drug resistance, resulting in poor prognosis. microRNA184 plays a role in many diseases such as tumor and inflammation, but whether microRNA184 regulates ovarian cancer cisplatin resistance is unknown. The ovarian cancer SKOV3 cell line and cisplatin-resistant strain cell SKOV3/DDP were cultured and microRNA184 expression was analyzed by Real time PCR. Transfection of microRNA184 siRNA into SKOV3/DDP under ly294002 (PI3K/AKT inhibitor) treatment followed by analysis of cell proliferation by MTT assay, Caspase 3 activity, cell migration by cell scratch assay, levels of Bax and Bcl-2 by Real time PCR and PI3K/AKT signaling by Western blot. microRNA184 expression was significantly increased in SKOV3/DDP cells compared to SKOV3 cells (P < 0.05). microRNA184 siRNA can significantly down-regulate microRNA184 expression in SKOV3/DDP, inhibit cell proliferation, increase Caspase 3 activity, inhibit cell migration, increase Bax expression, decrease Bcl-2 and decrease pAKT expression (P < 0.05). Addition of ly294002 further significantly promoted the above changes (P < 0.05). Targeting microRNA184 can inhibit SKOV3/DDP cell apoptosis by inhibiting PI3K/AKT signaling pathway, decrease tumor cell proliferation and migration, and inhibit the occurrence and development of cisplatin-resistant ovarian cancer.
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