MicroRNA-155 induces macrophage polarization to M1 in Toxoplasma gon-dii infection

Abstract

To investigate microRNAs differential expression and polarization of human macrophages in Toxoplasma gondii infection. The microRNAs differential expression of human macrophages in T. gondii infection was analyzed by microarray, and further validated by qRT-PCR. pEGFP-miR-155 was transfected into THP-1 cells by Lipofectamine M2000 and the transfection ratio was detected by flow cytometry. Flow cytometry was used to detect CD86 molecular on the macrophages. qRT-PCR was used to detect iNOS and IL12 mRNA expression. NO and IL12 expression were then evaluated by using ELISA. The miR-155 up-regulated more than 4-fold in T. gondii infected macrophages and enhanced as well as post-infection prolong. pEGFP-miR-155 transfection ratio was 82.6%.compared to cells cultured with T. gondii, pEGFP-miR-155 and miR-155-inhibitor, T. gondii and pEGFP-miR-155 inducement enhanced expression of CD86. Additionally, iNOS and IL12 mRNA were enhanced by qRT-PCR (P<0.05). NO and IL12 expression were increased by ELISA. T. gondii infection up-regulates the host miR-155 expression to modulate macrophages polarization to M1.