Abstract
MicroRNAs (miRNAs) influence stem cell functions, including mobilization, proliferation, and differentiation. miR-150 is abundantly expressed in monocytes. Knockdown of miR-150 promotes bone marrow stem cell migration. The role of miR-150 in adipose-derived stem cells (ADSCs) is unclear. In this study, the effects of miR-150 on adipogenic differentiation and proliferation of ADSCs were investigated. ADSCs were isolated from the inguinal adipose tissue of wild-type (WT) and miR-150 knockout (KO) mice and were induced for adipogenic differentiation. The miR-150 level was detected by real-time PCR. ADSCs were transfected by miR-150 or small-interfering RNA (siRNA) of Notch3. MTT assay and colony formation assay were performed in miR-150 knockdown and control ADSCs. Real-time PCR showed that miR-150 was expressed in ADSCs. miR-150 knockdown significantly decreased the capacity of adipogenic differentiation of ADSCs, as compared with their counterparts from WT mice. It is intriguing that the overexpression of miR-150 significantly increased C/EBPα and PPAR-γ expression and lipid formation in ADSCs with adipogenic induction. Overexpression of miR-150 significantly decreased Notch3 expression in ADSCs compared with the control groups. Furthermore, Notch3 inhibition promoted the adipogenic differentiation in ADSCs. miR-150 also suppressed proliferation potential and the expression of Nanog in ADSCs. In summary, this study demonstrates, for the first time, that miR-150 promotes adipogenic differentiation and inhibits proliferation of ADSCs. miR-150 regulates adipogenic differentiation of ADSCs, likely mediated by the downregulation of Notch3.
Highlights
adipose-derived stem cells (ADSCs) are a population of mesenchymal stem cells found in adipose tissue and are readily accessible from subcutaneous liposuction
The investigations show that ADSCs are a promising cell source for adipose tissue engineering as they can be differentiated into adipocytes [1]
We demonstrated for the first time that miR-150 played an important role in ADSC differentiation towards the adipose by targeting Notch3
Summary
ADSCs are a population of mesenchymal stem cells found in adipose tissue and are readily accessible from subcutaneous liposuction. MiRNAs play an important role in the posttranscriptional regulation of target mRNA in a range of biological processes, including maintenance of stemness and modulation of mobilization, proliferation, and differentiation of ADSCs [2]. Recent studies have reported that miR-150 is expressed in bone marrow mesenchymal stem cells (BMSCs). It was previously reported that control of the Notch signal through miR-150 might have an important impact on T cell development [15] It is not clear, whether miR-150 regulates the adipogenic differentiation of ADSCs by targeting Notch signaling. We investigated the mechanism of miR-150 in adipogenic differentiation of ADSCs. We demonstrated for the first time that miR-150 played an important role in ADSC differentiation towards the adipose by targeting Notch
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
