MicroRNA-124-3p inhibits cell growth and metastasis in cervical cancer by targeting IGF2BP1.

Abstract

MicroRNAs (miRs) serve a role in promoting and suppressing tumors in various types of malignant cancer, such as cervical cancer. However, the regulatory mechanism of miR-124-3p in cervical cancer remains unclear. In the present study, miR-124-3p was significantly downregulated in cervical cancer tissues and cell lines compared with matching adjacent non-tumor tissues and the normal cervical epithelial cell line End1/E6E7, respectively. Decreased expression of miR-124-3p was associated with advanced cervical cancer and the results of an in vitro study demonstrated that the ectopic expression of miR-124-3p significantly decreased the proliferation, migration and invasion of cervical cancer Caski cells. Furthermore, insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) was identified as a novel target of miR-124-3p. Overexpression of miR-124-3p decreased the expression of IGF2BP1, whereas miR-124-3p knockdown promoted IGF2BP1 expression at the post-transcriptional level in Caski cells. Additionally, overexpression of IGF2BP1 attenuated the suppressive effects of miR-124-3p on the proliferation, migration and invasion of Caski cells. IGF2BP1 was upregulated in cervical cancer tissues and cell lines compared with matching adjacent non-tumor tissues and the End1/E6E7 cell line, respectively. Therefore, the present study suggests that miR-124-3p suppresses the growth and metastasis of cervical cancer by directly targeting IGF2BP. Thus, miR-124-3p may be developed as a novel method of treating cervical cancer.