MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity.

S O Jaafar,S A Ibrahim,J O Jaffar,K K Jarjees

doi:10.3389/bjbs.2021.10207

S O Jaafar, S A Ibrahim + Show 2 more

Open Access

https://doi.org/10.3389/bjbs.2021.10207

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Abstract

Background: While different studies have investigated the association of SNPs with female reproductive disorders, a limited number of studies have investigated the effect of microRNAs variants in endometriosis. In this study, we evaluated the prevalence and the association of three different miRNAs variants including, miR-27a rs895819, miR-124-1 rs531564, and miR-423 rs6505162 with endometriosis to help further elucidate the importance of these variants in female reproductive disorders. Methods: A total number of 440 women (220 cases and 220 controls) were included. DNA was extracted and genotyping of the SNPs was carried out by PCR. Results: The results showed that rs895819 and rs6505162 had a significant association with endometriosis under the dominant, recessive, co-dominant, and allelic model, but rs531564 was not linked to endometriosis. Our results also imply a protective effect on endometriosis severity for AG genotype and G allele in rs895819 (p < 0.001), and also for AA and AC genotypes in rs6505162 with severity in endometriosis (p < 0.001). Moreover, Hardy–Weinberg equilibrium, haplotype frequency, and linkage disequilibrium between SNPs were performed. Conclusion: miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are linked to endometriosis.

Highlights

Infertility affects between 8 and 15% of couples of reproductive-age worldwide (1), the majority being residents of developing countries (2)
The study has been approved by the high graduate committees of the College of Medicine, Hawler Medical University, Erbil, Kurdistan Region-Iraq
The informed consent documents were obtained from all subjects prior to sampling

Summary

Introduction

Infertility affects between 8 and 15% of couples of reproductive-age worldwide (1), the majority being residents of developing countries (2). Researchers have shown a strong and consistent association between infertility and higher rates of endometriosis incidence among women with reproductive disorders and have considered endometriosis to be a leading cause of infertility (4). It is estimated that a quarter of reproductive-aged women suffer from endometriosis and accounts for up to 50% of female infertility cases (5). While different studies have investigated the association of SNPs with female reproductive disorders, a limited number of studies have investigated the effect of microRNAs variants in endometriosis. We evaluated the prevalence and the association of three different miRNAs variants including, miR-27a rs895819, miR-124-1 rs531564, and miR-423 rs6505162 with endometriosis to help further elucidate the importance of these variants in female reproductive disorders

Methods

Results

Conclusion