Abstract

This chapter describe the possibilities of MicroRNAs (miRNAs) in crop plants gene expression regulation in different metabolic pathways. Several current researches have shown different environmental stresses induce abnormal expression of miRNA, thus signifying that miRNAs may be an appropriate tool for genetical improvement in plant for stress tolerance. These miRNAs mainly control gene expression through translational inhibition. Generally, stress induce miRNAs-based inhibition of their target mRNAs, however, positive transcription factors accumulated and become more active after mRNA inhibition. Initially, researchers were mainly focused on miRNA identification, appropriate to specific or multiple environmental condition, expression profiling and recognize their roles in stress tolerance. Transformed miRNA expression studied in some plant species for better understanding of plant development and stress tolerance such as heavy metal, salinity, temperature, drought and nutrient deficiency. All these findings indicate that miRNAs act as a potential tool for genetic engineering and to enhance stress tolerance in crop plants.

Highlights

  • On the basis of current research, it describes the miRNA-based strategies used as potential tool for gene regulators in biofuel sources, beverages, cereals, fruits, fibers and economically important crops

  • One strand of the miRNA is inserted into AGO protein comprised RNA-induced silencing complex (RISC) complex, while other stand degraded by exosome along with small-RNA degrading nuclease [8]

  • Mentions exemplified case studies indicated that currently miRNAs viewed as a most essential gene regulator tool

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Summary

Introduction

Plants form an essential portion of the earth system and used by man as food, shelter, and a great source of medicine. Climate change and ever-increasing demand of plant products has the ability to modify the atmospheric properties and modify soils, which can make crop yield, development and growth more difficult. One of the important ways involves to enhance the yield and crop productivity is by using environmentally friendly plant protection measures [1]. 2. miRNA biosynthesis and their conservation in plants miRNA is ranges from 20 to 24 nucleotide base pair in length and non-coding RNA molecule [6]. One strand of the miRNA is inserted into AGO protein comprised RISC complex, while other stand degraded by exosome along with small-RNA degrading nuclease [8]. This miRNA promotes the RISC loading to complementary mRNAs targets. It showed that few miRNAs are lost during evolution [14]

Gene expression regulation by miRNA
Techniques used for miRNA isolation, identification, and characterization
Approaches for miRNA target screening and prediction
Functional roles of miRNAs
Improve crop tolerance to abiotic and biotic stresses using microRNA-based approaches
Conclusion and future perspectives
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