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Abstract
To understand the mechanism of small non-coding RNAs (miRNA)-mediated development and ripening of mulberry fruits, three small RNA libraries from mulberry fruits at different development stages were constructed, and 159 conserved miRNAs as well as 86 novel miRNAs were successfully identified. Among the miRNAs identified, there were 90 miRNAs which showed differential expression patterns at different stages of fruit development and ripening. The target genes of these differential expressed (DE) miRNAs were involved in growth and development, transcription and regulation of transcription, metabolic processes, and etc. Interestingly, it was found that the expression level of mul-miR477 was increased with fruit ripening, and it can target the antisense lncRNA (Mul-ABCB19AS) of the ATP binding cassette (ABC) transporter B 19 gene (Mul-ABCB19). Our results showed that mul-miR477 can repress the expression of Mul-ABCB19AS and increase the expression of Mul-ABCB19, and it acted as a positive regulator participating anthocyanin accumulation through the regulatory network of mul-miR477—Mul-ABCB19AS—Mul-ABCB19.
Highlights
MiRNAs are small non-coding RNAs, approximately 18–24 nucleotides in length, which modulate gene expression by cleaving or inhibiting the translation of their target genes (Yu et al, 2017)
It was showed that miR164 and miR169 can mechanism of small non-coding RNAs (miRNA) Profiling of Mulberry Fruit During Ripening inhibit the expression of NAC and NF-YA transcription factors and participated in fruit ripening, respectively (Li et al, 2017), and miR172 regulates fruit ripening through targeting the APETALA2a (AP2a) gene which negatively regulates ethylene biosynthesis and signaling (Karlova et al, 2011)
Three miRNA libraries (MG, MR, and MP) were constructed using the samples from three different development stages to explore the expression profiles of miRNAs during mulberry fruit development and ripening, and 159 conserved miRNA members belonging to 68 families were identified
Summary
MiRNAs are small non-coding RNAs, approximately 18–24 nucleotides in length, which modulate gene expression by cleaving or inhibiting the translation of their target genes (Yu et al, 2017). It was showed that miR164 and miR169 can miRNA Profiling of Mulberry Fruit During Ripening inhibit the expression of NAC and NF-YA transcription factors and participated in fruit ripening, respectively (Li et al, 2017), and miR172 regulates fruit ripening through targeting the APETALA2a (AP2a) gene which negatively regulates ethylene biosynthesis and signaling (Karlova et al, 2011). Apart from these conserved miRNAs, some non-conserved miRNAs, such as miR1917, miR4376, miR858, etc., were found to be associated with tomato fruit ripening (Christopher et al, 2020). Identification of conserved and species-specific miRNAs is of great significance for understand the molecular regulation mechanism of fruit development and ripening
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