Abstract
Synthetic DNAzyme motors or machines hold great potential in the detection of intracellular microRNA (miRNA) and mRNA. However, to make intracellular DNAzyme motors or machines operate efficiently, adding exogenous metal ion cofactors as fuel is imperative, which limits their applications. Here, we reported a Na+-specific DNAzyme-based DNAzyme motor differentiating cell subtypes of nonsmall cell lung cancer by simultaneously sensing intracellular miRNA-21 and miRNA-205. The DNAzyme motor could be fueled by intracellular Na+, which avoids the necessity of adding exogenous cofactors. It could be also designed to detect other miRNAs or mRNAs by changing 12-nt DNA domain. Meanwhile, our DNAzyme motor had high sensitivity, excellent specificity, high biostability, and little cytotoxicity. Therefore, the miRNA-initiated and intracellular Na+-fueled DNAzyme motor can expand the application of DNAzyme motors or machines in sensing miRNA and has potential value in cancer clinical diagnosis and prognosis.
Highlights
Analytical ChemistryThe Dmz-21 motor presented a low fluorescence background and high target-induced fluorescence signal, resulting in an ∼8fold fluorescence enhancement in the presence of 60 mM Na+ and 10 nM target DNA when the locking strand was designed to hybridize with the 12-nt ID domain plus seven bases of the left substrate-recognizing arm of Na+-specific DNAzyme (L21-7)
Synthetic DNAzyme motors or machines hold great potential in the detection of intracellular microRNA and mRNA
N umerous studies have confirmed that microRNAs play important roles in post-transcriptional regulation of gene expression.[1]. Their expression maladjustment is usually involved in the occurrence and development of cancer.[2]
Summary
The Dmz-21 motor presented a low fluorescence background and high target-induced fluorescence signal, resulting in an ∼8fold fluorescence enhancement in the presence of 60 mM Na+ and 10 nM target DNA when the locking strand was designed to hybridize with the 12-nt ID domain plus seven bases of the left substrate-recognizing arm of Na+-specific DNAzyme (L21-7). AuNPs. Dzms motor could simultaneously sense miRNA-21 and miRNA-205, resulting in an ∼6-fold enhancement in the fluorescence of Fam or Cy5 (Figure 4A). Control motors, which were prepared with Dzm-21 control 1 and control 2 (Table S1), could not yield FAM fluorescence signal in A549 cells due to lack of catalytic moiety or sensing moiety (Figure S8), suggesting intracellular Dzm motors had good specificity and low background noise.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
