Abstract
Gastric carcinoma (GC) refers to a common digestive system disease that exhibits a very high incidence. MicroRNA hsa-mir-3923 belongs to a type of miRNA, of which the function has been merely investigated in breast, pancreatic cancers and pre-neoplasic stages of gastric cancer. It has not been studied or reported in gastric carcinoma, so the relationship between gastric hsa-mir-3923 expression and the clinics feature and pathology of GC cases was examined. This study employed data mining for analyzing gastric carcinoma data in The Cancer Genome Atlas database. A Chi squared test was performed for assessing the relations of hsa-mir-3923 expression with clinics-related and pathology-regulated variables. This study conducted the assessment of the role of hsa-mir-3923 in prognostic process using Kaplan–Meier curves, Receiver operating characteristic (ROC) analysis and proportional hazards model (Cox) study. With the use of Gene Expression Omnibus, this study carried out gene set enrichment analysis (GSEA). In the meantime, the common miRNA database was compared to predict potential target genes; as revealed by co-expression analysis, a regulatory network probably existed, containing hsa-mir-3923. For the analysis of the most tightly associated cytological behavior and pathway in GC, this study adopted the databases for Annotation, Visualization and Integrated Discovery (David) and KO-Based Annotation System (KOBAS). Cytoscape, R and STRING were employed for mapping probable regulatory networks displaying relations to hsa-mir-3923. Lastly, we obtained 69 genes most tightly associated with hsa-mir-3923 and described their relationship with Circos plot. As revealed from the results, hsa-mir-3923 displayed up-regulation in gastric carcinoma, and it displayed associations with vital status, N stage and histologic grade when being expressed. The predicted results of miRNA target genes suggested that there may be a close relationship between 66 genes and hsa-mir-3923 in gastric cancer. As indicated from co-expression data, a small regulating network of 4 genes probably existed. Our results elucidated that hsa-mir-3923 high-expression reveals poor prognosis of GC patients.
Highlights
Gastric carcinoma (GC) refers to a common digestive system disease that exhibits a very high incidence
According to the recent study, Dr Li identified that lncRNA-NUTF2P3-001 could inhibit the pathway of hsa-mir-3923/KRAS affected by oxidative stress[7], which affected the proliferation of tumor; the role of hsa-mir-3923 as a specific marker in the gastric carcinoma should be explained
The target gene of this type of special miRNA was searched in numerous common miRNA databases first, followed by drawing the comparison of these data with those achieved from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO)
Summary
Gastric carcinoma (GC) refers to a common digestive system disease that exhibits a very high incidence. MicroRNA hsa-mir-3923 belongs to a type of miRNA, of which the function has been merely investigated in breast, pancreatic cancers and pre-neoplasic stages of gastric cancer It has not been studied or reported in gastric carcinoma, so the relationship between gastric hsa-mir-3923 expression and the clinics feature and pathology of GC cases was examined. MicroRNA can noticeably affect the study on cancer in terms of tumor’s biological activity (e.g., tumor transcription, epigenetic regulation, as well as expression of gene)[3]. They are capable of interacting with mRNA or lncRNA, leading to the formation of pathway in human cancers[4]. The paper discussed the biological activities hsa-mir-3923 participated and possible effects hsa-mir-3923 imposed
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