MicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection

Xuefeng Qi,Bo Yang,Qinghong Xue,Jingyu Wang,Ting Wang,Zhen Li

doi:10.1186/s13567-018-0565-3

Abstract

Peste des petits ruminants virus (PPRV) belongs to the genus Morbillivirus that causes an acute and highly contagious disease in goats and sheep. Virus infection can trigger the change in the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine cellular miRNA expression profile in goat peripheral blood mononuclear cells (PBMC) infected with Nigeria 75/1 vaccine virus, a widely used vaccine strain for mass vaccination programs against Peste des petits ruminants. Expression analysis demonstrated that PPRV infection can elicit 316 significantly differentially expressed (DE) miRNA including 103 known and 213 novel miRNA candidates in infected PBMC at 24 hours post-infection (hpi) as compared with a mock control. Target prediction and functional analysis of these DEmiRNA revealed significant enrichment for several signaling pathways including TLR signaling pathways, PI3K-Akt, endocytosis, viral carcinogenesis, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation of the roles of miRNA in PPRV replication and pathogenesis.

Highlights

Peste des petits ruminants (PPR) is an acute, highly contagious fatal disease in domestic and small wild ruminants, causing great economic losses in goat and sheep productivity [1]
Characterization of Peste des petits ruminants virus (PPRV) replication in goat peripheral blood mononuclear cells (PBMC) To determine the kinetics of PPRV replication in goat PBMC, cytopathic effect (CPE) and viral N protein expression were all detected at 0, 24, 48 and 72 hpi
PPRV- and mock-infected cells were harvested at 24 hpi in triplicates for further miRNA sequencing analysis to guarantee a higher proportion of infected cells and to avoid excessive CPE

Summary

Introduction

Peste des petits ruminants (PPR) is an acute, highly contagious fatal disease in domestic and small wild ruminants, causing great economic losses in goat and sheep productivity [1]. Peste des petits ruminants virus (PPRV), the causative agent of the disease, belongs to the genus Morbillivirus within the family Paramyxoviridae [2]. Live attenuated vaccines have already been used for control of PPR and showed a good immunological effect on both sheep and goats [6, 7]. Among these live attenuated vaccines, Nigeria 75/1 and Sungri/96 have been demonstrated to be safe and efficacious in conferring protection to sheep and goats

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Results

Conclusion