Abstract
MicroRNAs (miRNAs) are important regulators of gene expression, affecting many biological processes. As yet, their roles in the response of chrysanthemum to aphid feeding have not been explored. Here, the identity and abundance of miRNAs induced by aphid infestation have been obtained using high-throughput Illumina sequencing platform. Three leaf small RNA libraries were generated, one from plants infested with the aphid Macrosiphoniella sanbourni (library A), one from plants with mock puncture treatment (library M), and the third from untreated control plants (library CK). A total of 7,944,797, 7,605,251 and 9,244,002 clean unique reads, ranging from 18 to 30 nucleotides (nt) in length, were obtained from library CK, A and M, respectively. As a result, 303 conserved miRNAs belonging to 276 miRNAs families and 234 potential novel miRNAs were detected in chrysanthemum leaf, out of which 80, 100 and 79 significantly differentially expressed miRNAs were identified in the comparison of CK-VS-A, CK-VS-M and M-VS-A, respectively. Several of the differentially abundant miRNAs (in particular miR159a, miR160a, miR393a) may be associated with the plant's response to aphid infestation.
Highlights
Small RNAs, ranging in length from 18 to 40 nucleotides, regulate gene expression via their interaction with specific mRNA targets, thereby playing a significant role throughout plant growth and development [1, 2]
MicroRNAs, approximately 22 nt in size, are the most abundant species of endogenous noncoding small RNA (smRNA) in plants [3, 4]. They have been implicated in the response to both biotic and abiotic stress [5, 6], by guiding the cleavage or repressing the translation of their target mRNAs, which is based on nearly perfect complementarity with the targets, resulting in gene silencing at post-transcriptional levels [3, 7]
Three smRNA libraries were constructed from RNA extracted from pooled leaf material taken from chrysanthemum cultivar ‘Nannongxunzhang’ plants at various time points
Summary
Small RNAs (smRNAs), ranging in length from 18 to 40 nucleotides (nt), regulate gene expression via their interaction with specific mRNA targets, thereby playing a significant role throughout plant growth and development [1, 2]. MicroRNAs (miRNAs), approximately 22 nt in size, are the most abundant species of endogenous noncoding smRNAs in plants [3, 4]. They have been implicated in the response to both biotic and abiotic stress [5, 6], by guiding the cleavage or repressing the translation of their target mRNAs, which is based on nearly perfect complementarity with the targets, resulting in gene silencing at post-transcriptional levels [3, 7]. Evidence from the discovery that miR398 targeting two Cu/Zn superoxide dismutases (CSD1 and CSD2) and a copper chaperone for CSD1 responds to both oxidative stress
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