Abstract
BackgroundMicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. However, few studies have focused on the effects of miRNAs on early human embryonic development. The relationship between miRNAs and early human embryogenesis is still unknown.ResultsIn this study, RNA-seq data collected from sperm samples from 102 patients with a normal sperm index but treated with assisted reproductive technology (ART) were analyzed for the relationships between differentially expressed small RNAs and the fertilization rate (FR), blastocyst rate and high-quality embryo rate (HQER). The sperm samples with high hsa-mir-191 expression had a higher FR, effective embryo rate (EER) and HQER. hsa-mir-191 was used as a single indicator to predict the HQER. The receiver operating characteristic (ROC) curve had an area under the ROC curve (AUC) of 0.686. We also found that hsa-mir-191 expression is correlated with an abnormal sperm rate (cor = 0.29, p < 0.01). We also evaluated the relationship between hsa-mir-34c and early human embryo development in these 102 sperm samples and obtained negative results.ConclusionsThese findings suggest that high hsa-mir-191-5p expression in sperm is associated with early human embryonic quality and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).
Highlights
MicroRNAs are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression
We evaluated the role of the controversial miR-34 family, which comprises the most previously reported sperm-carrying miRNAs that play an important role in the early embryonic development of mice/bovines following in vitro fertilization (IVF) in humans
Overview of small RNA library sequencing in spermatozoa and sample grouping To evaluate the role of miRNAs in spermatozoa in the process of embryo development during IVF, miRNA profiles in the spermatozoa of 102 patients who underwent IVF were investigated by small RNA deep sequencing
Summary
MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. Few studies have focused on the effects of miRNAs on early human embryonic development. There are few studies on the relationship between miRNAs and early human embryo development. Studies suggested that sperm played a role only in transmitting the paternal genome during the reproductive process. An increasing number of studies have shown that the role of sperm in the reproductive process is diversified. The sperm content contains many coding and noncoding RNAs [4] that play an important role in epigenetics [5, 6]
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