MicroRNA-96-5p promotes proliferation, invasion and EMT of oral carcinoma cells by directly targeting FOXF2.

Haiyan Wang,Wenyue Li,Zuomin Wang,Ning Ma

doi:10.1242/bio.049478

Haiyan Wang, Wenyue Li + Show 2 more

Open Access

https://doi.org/10.1242/bio.049478

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Abstract

ABSTRACTRecently, microRNA-96-5p (miR-96-5p) has been reported to function as both a tumor suppressor and oncogene in several cancer types, including gastric cancer, hepatocellular cancer and lung cancer. However, the biological function of miR-96-5p and its precise mechanisms in oral squamous cell carcinoma (OSCC) have not been well clarified. The aim of this study was to study the roles of miR-96-5p/FOXF2 axis in OSCC. In this study, the miR-96-5p level was dramatically enhanced in OSCC tissues and cell lines, and the FOXF2 expression was significantly reduced. In addition, the FOXF2 expression was negatively related to the miR-96-5p level in OSCC tissues. Furthermore, downregulation of miR-96-5p obviously restrained OSCC cell proliferation, invasion and EMT. We confirmed that miR-96-5p could directly target FOXF2 by luciferase reporter assay. Moreover, knockdown of FOXF2 also could markedly promote the proliferation, invasion and EMT of OSCC cells. Finally, overexpression of FOXF2 in OSCC cells partially reversed the promoted effects of miR-96-5p mimic. Knockdown of miR-96-5p restrained OSCC cells proliferation, invasion and EMT via regulation of FOXF2.

Highlights

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide
The effect of miR-96-5p on proliferation of oral squamous cell carcinoma (OSCC) cells After transfection with miR-96-5p mimic or inhibitor, the results showed that the miR-96-5p level was significantly upregulated or downregulated in a miR-96-5p mimic or inhibitor group compared to a negative control (NC) group (Fig. 2A), respectively
Numerous studies have found that miR-96-5p affected cell proliferation, but was closely associated with prognosis in cancers including ovarian cancer, HNSCC, hepatocellular carcinoma and colorectal cancer (Liu et al, 2019; Vahabi et al, 2019; Iwai et al, 2018; Ress et al, 2015)

Summary

Introduction

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide. It is reported that 1.6 million new cases of HNSCC are diagnosed each year, and half of HNSCC is oral squamous cell carcinoma (OSCC) with 333,000 deaths (Warnakulasuriya, 2009). There are several therapeutic treatments such as chemotherapy combined with radical surgery and surgery combined with radiation, the 5-year survival rate of OSCC is only approximately 50% (Leemans et al, 2011). The pathogenesis of OSCC is complex, and many genes and pathways are involved in it. MicroRNAs (miRNAs) are a family of small, endogenous noncoding RNAs. MicroRNAs (miRNAs) are a family of small, endogenous noncoding RNAs They regulate the translation or induce degradation of specific protein coding genes through binding to the 3′-untranslated regions of the mRNA (Ambros, 2004). It was predicted that miRNAs targeted more

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