Abstract
BackgroundMicroRNA (MiRNA) is a small non-coding RNA which is implicated in a cohort of biological function in cancer, including proliferation, metastasis, apoptosis and invasion. MiR-96 has been reported to be involved in many cancers, including papillary thyroid cancer. However, the role of miR-96-3p in papillary thyroid cancer metastasis is still unclear.MethodsqRT-PCR is used to detect the level of miR-96-3p and mRNA of SDHB in PTC tissues and cell lines. Western blot assays are used to verify the protein expression of SDHB. The transwell assays are performed to identify the migration ability of PTC cell lines. Moreover, dual-luciferase 3′-UTR reporter assays are chosen to illuminate the direct target of miR-96-3p.ResultsThe relative miR-96-3p upregulate in PTC tissues and three PTC cell lines (B-CPAP, K-1 and TPC-1 cells) while the relative SDHB is opposite. Our results revealed that the miR-96-3p promotes metastasis and invasion in PTC cell lines (K-1 and TPC-1 cells) by direct targeting SDHB and influence the downstream protein AKT.ConclusionsTaken together, the miR-96-3p is involved in PTC metastasis and invasion by direct targeting SDHB and the downstream molecule AKT and mTOR.
Highlights
MicroRNA (MiRNA) is a small non-coding RNA which is implicated in a cohort of biological function in cancer, including proliferation, metastasis, apoptosis and invasion
MiR‐96‐3p in human Papillary thyroid cancer (PTC) tissues compared with paired adjacent normal tissues and three PTC cell lines is significantly up‐regulated To explore the function of miR-96-182-183 cluster in PTC, we collected clinical data and the corresponding PTC tissues and paired adjacent normal tissues of the 28 PTC patients
We determined whether the miR-96-3p was overexpression in the PTC cell lines (B-CPAP, K-1 and TPC-1 cells)
Summary
MicroRNA (MiRNA) is a small non-coding RNA which is implicated in a cohort of biological function in cancer, including proliferation, metastasis, apoptosis and invasion. MiR-96 has been reported to be involved in many cancers, including papillary thyroid cancer. The role of miR-96-3p in papillary thyroid cancer metastasis is still unclear. PTC has been reported to be accounted for the most cases among the thyroid cancers, which is regularly found in women and children [1, 2]. The increasing incidence of papillary thyroid cancer has been nearly universal in recently years [3,4,5]. Papillary thyroid cancer is the malignancy with fastest rising incidence in some regions. Among papillary thyroid cancers who are undertaken the surgery are generally associated with a favorable prognosis, the rate of recurrence can be still in 10–15% [4, 5].
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