MicroRNA-93 regulate proliferation and apoptosis of renal cancer cells by targeting large tumor suppressor homolog 2

Abstract

Objective To evaluate the effect of microRNA (miRNA, miR)-93 regulate proliferation and apoptosis of renal cancer cells by targeting large tumor suppressor homolog 2 (LATS2). Methods Chose renal cancer cells line GRC-1, and used dual-luciferase report gene experiment to verification the targeted relationship between miR-93 and LATS2. Respectively transfected Neg-miR, pre-miR-93 and anti-miR-93 to GRC-1 cells. The miR-93 expression was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The LATS2 protein expression was detected by Western blotting. The cell proliferation activity was detected by methyl thiazol tetrazolium (MTT) method. The cell apoptosis were detected by flow cytometry. Results Between miR-93 and LATS2 had some binding sites. Compared with transfection Neg-miR, co-transfection pre-miR-93 and LATS2-wt can made luciferase activity of GRC-1 significantly decreased (P=0.000). Compared with Neg-miR group, the miR-93 expression of pre-miR-93 group significantly increased (P=0.000), and the LATS2 protein expression significantly decreased (P=0.000). The miR-93 expression of anti-miR-93 group significantly decreased (P=0.000), and the LATS2 protein expression significantly increased (P=0.000). Compared with Neg-miR group, the proliferation activity of pre-miR-93 group significantly increased (P=0.000), and the apoptosis rate of significantly decreased(P=0.000). The proliferation activity of anti-miR-93 group significantly decreased(P=0.000), and the apoptosis rate of significantly increased (P=0.000). Conclusion miR-93 can regulate proliferation and apoptosis of renal cancer cells by targeting LATS2. Key words: MicroRNA-93; Large tumor suppressor homolog 2; Renal cancer; Proliferation; Apoptosis