Abstract
BackgroundMicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression.MethodsThe levels of miR-663 in ovarian cancer cell lines and clinical tissues were detected using qRT-PCR assays. The Transwell invasion and wound healing assay were conducted to assess the roles of miR-663 in the migration and invasion of ovarian cancer cell in vitro. Rescue assays were carried out to confirm the contribution of tumor suppressor candidate 2 (TUSC2) in the aggressiveness of cancer cell which was regulated by miR-663.ResultsThe levels of miR-663 were up-regulated in ovarian cancer tissues in comparison with the corresponding normal tissues. Up-regulation of miR-663 increased the proliferation, colony formation, migration and invasion of ovarian cancer SKOV3 cell. Additional, over-expression of miR-663 increased the tumor growth of SKOV3 in xenograft model. Bioinformatics analysis and luciferase reporter assay identified that miR-663 decreased the level of TUSC2 via binding to the 3′-UTR of TUSC2 gene. Finally, the expression of TUSC2 was inversely associated with the level of miR-663 in ovarian carcinoma tissue and over-expression of TUSC2 inhibited the migration and invasion abilities of SKOV3 that was promoted by miR-663.ConclusionAltogether, these results indicate that miR-663 acts as a potential tumor-promoting miRNA through targeting TUSC2 in ovarian cancer.
Highlights
MicroRNAs have emerged as the critical modulators of the tumorigenesis and tumor progression
We found that the tumor growth was increased in mice that was inoculated with miR-663 transfected cells than that in the miR-negative control (miR-NC) group (Fig. 2d)
The Ki67 staining in tumor tissue that was formed by miR663 transfected SKOV3 cell was remarkably increased than that in the tumor tissue that derived from miR-NC transfected SKOV3 cell (Fig. 2e)
Summary
MicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression. MiRNAs function as tumor suppressor or oncogenic miRNA in the tumorigenesis and cancer progression based on the functions of their target genes [11]. Previous studies have shown the role of miR-663 in many important pathological processes, including autoimmune diseases, infection, and inflammatory reaction [12, 13]. Its function in tumor progression is contradictory. It acts as a cancer promoter in breast cancer and nasopharyngeal carcinoma, miR-663 may be shown as a potential tumor suppressor in glioblastoma and gastric cancer [14, 15]. To our knowledge, no functional evidence of miR663 in ovarian carcinoma has been documented
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