Abstract
Background Although studies manifested that microRNA-603 plays a vital role in many cancers, the modulatory mechanism of microRNA-603 in cutaneous melanoma remains unknown. We aimed to investigate the roles of microRNA-603 in cutaneous melanoma cells. Methods First, microRNA-603 expression in cutaneous melanoma cell lines was detected by qRT-PCR. The mRNA and protein expression levels of TBX5 in cutaneous melanoma cell lines were tested by qRT-PCR and western blot, respectively. In addition, the interaction between microRNA-603 and TBX5 was determined by dual-luciferase reporter gene assay, and their impacts on the growth of cutaneous melanoma cells were detected by cellular function experiments such as MTT, colony formation, and Transwell assays. Results The expression level of microRNA-603 in human cutaneous melanoma cells was relatively upregulated. Overexpressing microRNA-603 could promote progression of cutaneous melanoma cells, while silencing microRNA-603 expression could suppress the malignant progression of cutaneous melanoma. In addition, TBX5 was lowly expressed in cutaneous melanoma cells. As confirmed by dual-luciferase assay, microRNA-603 could specifically bind to 3′UTR of TBX5 and regulate TBX5. The results of the rescue experiment demonstrated that inhibiting microRNA-603 expression could suppress the proliferation, migration, and invasion of cutaneous melanoma cells, but its suppressive effect could be restored by TBX5. Conclusion MicroRNA-603 could regulate the expression of TBX5, thus promoting the malignant progression of cutaneous melanoma cells.
Highlights
MicroRNAs are a class of endogenous small noncoding RNA molecules, which play a negative role in regulating gene expression by binding to 3′ untranslated region (UTR) of the target mRNA to induce the translation inhibition or posttranscriptional degradation of mRNA [1, 2]
The results unveiled that microRNA-603 in human cutaneous melanoma cell lines was higher than that in the normal human melanoma cell line (Figure 1)
Melanoma cells Malme-3M with the lowest microRNA-603 expression level and A375 with the highest microRNA-603 expression level were selected for subsequent cellular function experiments
Summary
MicroRNAs are a class of endogenous small noncoding RNA molecules, which play a negative role in regulating gene expression by binding to 3′ untranslated region (UTR) of the target mRNA to induce the translation inhibition or posttranscriptional degradation of mRNA [1, 2]. Studies manifested that microRNA-603 plays a vital role in many cancers, the modulatory mechanism of microRNA-603 in cutaneous melanoma remains unknown. MicroRNA-603 expression in cutaneous melanoma cell lines was detected by qRT-PCR. The mRNA and protein expression levels of TBX5 in cutaneous melanoma cell lines were tested by qRT-PCR and western blot, respectively. The interaction between microRNA-603 and TBX5 was determined by dual-luciferase reporter gene assay, and their impacts on the growth of cutaneous melanoma cells were detected by cellular function experiments such as MTT, colony formation, and Transwell assays. The results of the rescue experiment demonstrated that inhibiting microRNA-603 expression could suppress the proliferation, migration, and invasion of cutaneous melanoma cells, but its suppressive effect could be restored by TBX5. MicroRNA-603 could regulate the expression of TBX5, promoting the malignant progression of cutaneous melanoma cells
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