Abstract
BackgroundStudies have shown that the decrease of osteogenic differentiation of bone marrow mesenchymal stem cells (MSC) is an important mechanism of osteoporosis. The object of this study was to explore the role and mechanism of microRNA miR-425-5p in the differentiation of MSC.MethodsThe expression of miR-425-5p in MSC was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Cell proliferation, cell cycle and apoptosis were detected by CCK-8 colorimetry and flow cytometry. The expression of TNF were detected by ELISA.ResultsOur data show that MiR-425-5p could modulate TNF-induced cell apoptosis, proliferation, and differentiation. ANXA2 is also the target of miR-425-5p and ANXA2 was involved in TNF-induced MSC cell apoptosis, proliferation, and differentiation. In addition, MiR-425-5p enhanced osteoporosis in mice.ConclusionMiR-425-5p might serve as a potential therapeutic target for the treatment of osteoporosis.
Highlights
Osteoporosis is a common metabolic bone disease characterized by inhibited bone mass, inhibited bone densities and bone microstructural destruction [1, 2]
Studies have found that the reduction of mesenchymal stem cells (MSC) to osteogenic differentiation is an important mechanism for the pathogenesis of osteoporosis [8]
MiR-425-5p could modulate TNF-induced cell apoptosis, proliferation, and differentiation To investigate the role of miR-425-5p, the expression of miR-425-5p was firstly evaluated in TNF treated MSCs
Summary
Osteoporosis is a common metabolic bone disease characterized by inhibited bone mass, inhibited bone densities and bone microstructural destruction [1, 2]. Mesenchymal stem cells (MSC) are one type of stem cells derived from mesoderm [6]. They have multi-directional differentiation potential and exist in various tissues such as the liver, skin, and placenta [7]. Studies have found that the reduction of MSC to osteogenic differentiation is an important mechanism for the pathogenesis of osteoporosis [8]. Studies have shown that the decrease of osteogenic differentiation of bone marrow mesenchymal stem cells (MSC) is an important mechanism of osteoporosis. Results: Our data show that MiR-425-5p could modulate TNF-induced cell apoptosis, proliferation, and differentiation. ANXA2 is the target of miR-425-5p and ANXA2 was involved in TNF-induced MSC cell apoptosis, proliferation, and differentiation. Conclusion: MiR-425-5p might serve as a potential therapeutic target for the treatment of osteoporosis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
