Abstract
Background Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. The abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. However, the function and the underlying mechanism of miR-384 in PTC progression remain largely unknown. Methods Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues. MTT, soft agar assay Transwell assay, and wound-healing assay were carried out to explore the biological function of miR-384 in PTC cell lines of BCPAP and K1. Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR-384. Moreover, Spearman's correlation analysis was conducted to reveal the correlation between miR-384 and PRKACB mRNA in PTC. Results The expression of miR-384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size. The ectopic upregulation of miR-384 significantly suppressed PTC progression, and the inhibition of miR-384 had the opposite effects. Moreover, PRKACB gene was confirmed as the target of miR-384. Conclusion The study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3′-UTR of PRKACB gene.
Highlights
Papillary thyroid cancer (PTC) is the most common subtype of thyroid malignancy with approximately 77% diagnosed in women [1]
Conclusion. e study suggests that miR-384 serves as a tumor suppressor in papillary thyroid cancer (PTC) progression by directly targeting the 3′-untranslated region (3′-UTR) of protein kinase catalytic beta subunit (PRKACB) gene
All the cases had no chemotherapy, radiotherapy, and immunotherapy history. e samples had been diagnosed and divided into PTC and adjacent noncancerous by two independent pathologists who were blinded to the clinical results on the basis of hematoxylin-eosin (HE) staining. e medical records including the age, gender, tumor size, and lymph node metastasis of the patients were collected. e study had been approved by the Ethics Committee of Xinxiang Medical University (Xinxiang, China)
Summary
Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. e abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. E abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. The function and the underlying mechanism of miR-384 in PTC progression remain largely unknown. Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues. Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR-384. Spearman’s correlation analysis was conducted to reveal the correlation between miR-384 and PRKACB mRNA in PTC. E expression of miR-384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size. PRKACB gene was confirmed as the target of miR-384. E study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3′-UTR of PRKACB gene Conclusion. e study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3′-UTR of PRKACB gene
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