MicroRNA‑383‑5p inhibits the proliferation and promotes the apoptosis of gastric cancer cells bytargeting cancerous inhibitor of PP2A.

Abstract

The aberrant expression of microRNA (miRNAor miR)‑383‑5p has been found in numerous types of cancer. However, the function of miR‑383‑5p in gastric cancer (GC) remains elusive and requires further investigation. In the present study, the level of miR‑383‑5p and cancerous inhibitor of PP2A (CIP2A) in GCcell lines was determined by reverse transcription‑quantitative PCR analysis. GCcell proliferation, apoptosis and cell cycle distribution were determined by the MTTassay and flow cytometry, respectively. The mRNA target of miR‑383‑5p was identified by dual luciferase activity assay. It was observed that the expression of miR‑383‑5p was lower and that of CIP2A was higher in GCcells compared with the GES‑1 normal human gastric epithelial cell line. Transfectoin with miR‑383‑5pmimic significantly inhibited GCcell proliferation, while it promoted cell apoptosis and G0/G1arrest by targeting CIP2A. Taken together, the findings of the present study demonstrate that miR‑383‑5p inhibits GCcell proliferation and promotes apoptosis and G0/G1arrest by targeting CIP2A, indicating that targeting miR‑383‑5p may hold promise as a future therapeutic strategy for patients with GC.