Abstract
Objective To study the effect of microRNA (miRNA)-374a on proliferation and migration of breast cancer cells and regulation of monocyte chemoattractant protein-1 (MCP-1).Methods Construct miRNA-374a over-expression MDA-MB-231 breast cancer cells,374a-PLL3.7-MDAs and noload plasmid MDA-MB-231 breast cancer cells,EV-PLL3.7-MDAs by slow virus plasmid PLL3.7; Detect the expression of miRNA-374a by reverse transcriptase-polymerase chain reaction (RT-PCR).Results miRNA-374a can significantly inhibit breast cancer cell proliferation,migration ability.Cell counting kit-8 (CCK-8) cells proliferation assays and monoclonal formed assays show that 374a-PLL3.7-MDAs proliferation was inhibited than EV-PLL3.7-MDA.Transwell chambers Experimental results show that the chamber film surface of 374a-PLL3.7-MDAs was significantly less than EV-PLL3.7-MDAs.Expression of MCP-1 was inhibited in 374a-PLL3.7-MDAs.Conclusion miRNA-374b may inhibit proliferation and migration of breast cancer cells by MCP-1. Key words: MicroRNA; Monocyte chemoattractant protein-1 ; Breast cancer; Proliferation; Migration
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